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It has recently been demonstrated that inflammation modulates vascular permeability. Here, we report studies evaluating the ability of leukocytes, platelets, their complexes, and pericardial fluid to stimulate vascular permeability. In the rabbit hemostatic chamber preparation, leukocytes (2.5-10 x 10(7)/ml) but not platelets (1.0 x 10(7) cells/ml) significantly stimulated exudate formation over a 6-hr incubation (p less than 0.05). Leukocyte-platelet complexes (2.5 x 10(7) cells/ml) induced a significant increase in vascular permeability over a 6-hr incubation (p less than 0.05). The quantity of leukocyte-platelet complexes, rather than their density, correlated with the magnitude of the vascular permeability. A similar increase in vascular permeability was observed when pericardial fluid (5 ml) was added to the chamber over a 6-hr incubation (p less than 0.05). Because the total protein content in leukocyte-platelet complexes and pericardial fluid was equivalent to the plasma protein content, these results suggest that vasoactive factors contained in the complex or pericardial fluid cause the increased vascular permeability. Pericardial fluid, isolated by sequential filtration on 0.2- and 3-microns membrane filters, did not stimulate increased exudate formation. Evidence for the presence of vasoactive compounds in the pericardial fluid includes two findings
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